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How will adjusting the split line affect my volume recovery?

Guidelines for theoretical volume recovery following levitation.

Adjusting the split line can be a valuable approach to increasing cell viability or cell yield. However, adjusting the split line will affect how much volume is recovered in the sample output well. Furthermore, the amount of volume collected can also be influenced by overall sample quality including total number of cells, amount of debris, viscosity of the sample, etc. See the table below for theoretical recovery volume based on split line. If a user needs a precise calculation of volume recovered from the cartridge, please visit this article.