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      LeviCell 96 - General FAQ

      FAQ for the LeviCell 96 System

      Frequently Asked Questions: LevaCell 96 System

      • What is the LevaCell 96 System and what does it do?
      • The LevaCell 96 System is a high-throughput cell enrichment and washing platform that utilizes levitation technology. It provides cell enrichment, cell washing, debris removal, and targeted selection in a single step. This enables faster sample preparation, improved data quality, and reduced costs compared to traditional methods.
      • What are the key benefits of using the LevaCell 96 System?
      • The LevaCell 96 System offers several key advantages:
      • Higher Throughput: Processes up to 96 samples in less than 50 minutes.
      • Lower Cost: Significantly reduces the cost per sample compared to traditional methods (e.g., $10 vs >$100).
      • More Data: Achieves 5X more viable cells, 10X less myelin and debris, and >99% targeted cell removal.
      • Scalability: Provides standardized, scalable, and reproducible processes.
      • Simplicity: Offers a fast, simple, and efficient workflow with fewer hands-on steps.
      • Sample Flexibility: Accommodates both adherent and non-adherent cells, from bacteria to organoids, and is tolerant of debris and contaminants.
      • How does the LevaCell 96 System work?
      • The LevaCell 96 System workflow involves three main steps:
      1. Add Levitation Agent: A levitation agent is dispensed into the 96 wells, along with any assay of interest.
      2. Levitate: The cells levitate based on their properties (viability, interactions, etc.) for 20-60 minutes.
      3. Collect: The desired fraction of cells is collected for downstream applications like sequencing, culture, or PDX.
      • What applications are suitable for the LevaCell 96 System?
      • The LevaCell 96 System is suitable for a wide range of applications, including:
      • Cytometry (incorporating pre-sorting enrichment, cell washing, and background removal)
      • CITE-Seq (pre-encapsulation enrichment, debris removal, cell washing, and background removal)
      • scRNA-Seq (pre-encapsulation enrichment, debris removal, cell washing, and background removal)
      • Primary Cell Dose Response
      • Organoid Testing
      • Cell-Cell Interaction studies
      • What type of samples can be processed with the LevaCell 96 System?
      • The LevaCell 96 System offers sample flexibility and can be used with a variety of sample types including:
      • Non-adherent and adherent cells
      • Bacteria
      • Organoids
      • Tumor samples
      • Multi-cell type samples
      • Plant cells
      • How does the LevaCell 96 System compare to traditional cell processing methods?
      • The LevaCell 96 System offers significant improvements over traditional methods:
      • Time and Steps Savings: Reduces hands-on steps and total processing time by up to 100-fold.
      • Improved Data Quality: Delivers superior data with increased cell viability, reduced debris, and unbiased cell population representation.
      • Reduced Cost: Offers a lower cost per sample.
      • Standardization: Eliminates technical variance, method and reagent variations, and operator bias associated with manual methods.
      • What are the system specifications of the LevaCell 96 System?
      • Key system specifications include:
      • Maximum number of samples per plate: 96
      • Volume per sample: 50 μL
      • Minimum number of input cells: 10,000 per well
      • Maximum number of input cells: 500,000 per well (48M cells total per plate)
      • Number of output fractions: 192
      • Enrichment time (typical protocol): 45 min
      • What is LeviSelect, and how does it integrate with the LevaCell 96 System?
      • LeviSelect refers to targeted cell selection, which is a capability of the LevaCell 96 system. This feature allows for the antibody-mediated removal of unwanted cells or contaminants during the washing process. The integration of LeviSelect enables simultaneous target cell and viable cell enrichment with significant performance improvements compared to market alternatives. This approach helps to remove unwanted cell populations (e.g., CD45+ cells in cancer samples), leaving cells of interest untouched for downstream analysis, thus yielding faster, more sensitive, and less expensive experiments.