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Can I separate neural cells from myelin?

Guidelines for enriching viable cells from dissociated neural tissue.

The short answer is potentially. Various neural cell types including microglia, astrocytes, and neurons have been successfully enriched via levitation. However, abundance of myeline may negatively impact viable cell levitation. This is due to general stickiness of samples with myelin, as well as the high lipid content of myelin which would impact cellular levitation profile. Please see our protocol on preparing mouse neural tissue prior to levitation.